Search for:

MITOMAP: Polymorphic MtDNA Restriction Sites Detected by High Resolution Screening of Human Populations

Last edited Jun 01, 2003
Enzyme Cut Sequence High Resolution Restriction Sites References
Acc I GTVWAC +13635, ~+14550, -15254, -15254 references
Alu I AGCT +259, +675, -856, -1240, +1403, -1610, -1670, +1718, -1893, -1917, -2208, +2223, +2250, +2293, +2384, +2734, -3537, +3981, -4310, -4411, -4631, -4685, -4769, +4877, -4990, +5133, -5176, -5584, -5697, -5823, -5978, -5996, -6022, -6204, -6867, +7025, -7055, -7474, -7641, -8074, +8198, +8268, +8327, +8466, +8484, +8678, +8678, +8774, +9009, +9299, +9504, -9644, +10028, +10097, +10135, +10143, -10352, +10397, +10413, -10598, +10694, +11100, +11321, +11350, -11362, +11425, +11469, -11576, +11806, +11892, -12282, -12560, +12763, +12990, +13068, +13262, +13284, -14015, -14015, -14304, -14304, +14322, +14509, +14899, +15245, +15437, +15606, -15776, +16226, +16240, +16246, +16254 references
Ava II GGRCC +748, +1830, ~+3876, ~+4280, +4308, +4332, ~+4346, +4432, +4481, ~+4776, +4811, ~+5229, +5259, +5260, +5984, ~+6000, +6324, +6332, ~+6382, +6581, +6699, ~+6700, +7025, -7055, +7804, ~+8229, +8249, +8249, ~+8270, +8391, +8391, ~+8400, +9589, +10028, -11577, ~+12130, +12191, -12629, -12629, -13367, -13367, +14258, ~+14400, ~+14900, +15487, +15487, ~+15490, +15499, +15591, +15882, +15882, ~+15890, -16390, -16390, +16503 references
BamHI GGATCC +13366, +13366, -14258, -14258, +16389, +16389, +16491 references
Bfa I CTAG +5004 references
BstNI CCRGG +13467, -13704, +14276 references
Dde I CTNAG +29, +64, -853, +868, +1043, -1637, -1667, -1715, -1923, +2247, -2856, -3192, +3388, -3534, +3846, +3930, -5003, +5076, -5552, -5646, +5671, -6296, +6356, -6377, -7103, +7319, -7750, -8309, -8515, +8569, +8714, +9147, -9272, -9500, -9641, +10394, -10631, +10746, +11074, +11146, +11793, -12663, -12891, +12946, -13065, +13467, +14385, +14394, +14493, -14608, +14773, +14923, -15073, -15238, -15250, +15434, +15660, +15727, +15751, -15996, +16262, +16270, +16297, -16380, +16467, +16479, +16528 references
EcoR I GAATTC No polymorphic sites detected references
FnuD II CGCG +14678 references
Hae II PGCGCQ +1622, -4529, +4830, -9052, +9326, +11001, +11968, +12949, +14223, -14858, -15002 references
Hae III GGCC +8, -322, +625, +663, +929, +1063, -1463, -1484, +1718, +2636, -3315, +3391, -3412, +3624, +3624, +3714, +3744, +3842, -3849, +4092, -4563, +4793, -4848, -5261, +5315, -5837, -6027, -6260, +6425, +6534, +6618, -6957, +7325, +7347, -7497, +7607, +7792, -7853, +7979, +8148, +8165, -8250, -8391, -8572, -8838, +8872, +8901, +8920, -8994, -9025, +9156, +9181, +9209, +9253, -9266, -9294, -9342, +9386, -9553, +9714, +9810, -9953, +10097, -10364, -10689, +10725, +11092, +11313, +11329, +11390, +12185, +13018, -13051, +13284, +13633, -13702, -13957, +14279, +14749, +14899, -15047, -15073, -15172, +15431, +15520, +15595, +15614, -15883, +16145, +16318, +16398, -16456, +16517, +16534 references
Hha I GCGC -160, +255, +1536, +1623, +1941, -3698, -4360, +4831, +5351, +5538, -5971, +6166, -7598, +7617, +7828, +8858, -9053, +9192, +9327, -9380, +10066, +11002, -11691, +11969, +12940, +12950, -13208, +13940, +14224, -14859, -15003, +15615 references
Hinc II GTQPAC +207, -1004, +3659, +3759, -7853, +7937, +12026, -12406, -13259, -13634, -14199, +14648, +16216 references
Hind III AAGCTT No polymorphic sites detected references
Hinf I GANTC +316, +717, +3359, +4092, +4546, -4810, +5072, +5198, -5983, -6211, +6610, -6871, -6931, +7672, +7970, +8615, -8783, +9209, +9493, +9683, -9753, +9820, +9859, +9984, +10054, -10256, +10806, -10830, -10971, -11403, +12008, -12170, +12925, -13031, -13103, -13268, -13916, +14268, -14368, +15005, -15234, -15375, -15723, -16000, -16065, +16096, +16246, +16389, +16490 references
Hpa I GTTAAC +207, +1004, +3592, +12026, -12406 references
Mae III GTNAC -11651 references
Mbo I GATC +8, +125, +340, -740, -951, +2113, +2349, +2390, +3090, -3569, +4026, +5372, +5389, -6904, +7316, +7570, -7658, +7852, -7859, +7933, +8565, -8592, -8616, -8729, +9150, +9942, +9985, -10254, +10934, +11431, +11439, -11922, +12171, +12528, +12629, +12795, +12849, +13004, +13104, +13152, +13180, +13367, +13575, -14259, +14279, +14749, -14869, -15060, +15195, +15235, +15397, -15591, +15790, +15954, +16145, +16170, +16176, +16215, +16373, +16390, +16398 references
Mse I TTAA -14766, +15904 references
Msp I CCGG +64, -104, -931, +4593, -4711, -5742, +5754, +6501, -6688, +7159, -8112, -8150, +11161, -11688, -12123, +13100, +14139, +14567, +15485, +15912, -15925, -16453, +16494 references
Nla III CATG +4336, -4577 references
Pst I CTGCAG -6910 references
Pvu II CAGCTG -1002 references
Rsa I GTAC +54, +163, -1307, -1476, +1830, +1842, -2758, -2849, -3123, -3337, +3371, +3397, +3987, +4051, -4464, +4643, +4723, +4732, +4745, -5054, +5164, +5492, +5566, +5985, +6915, -7013, +7241, +7697, +7702, -7897, -7912, -8012, +8078, +8156, +8269, +8299, +9336, +9429, -9746, +9926, +10407, +10524, +10644, +10656, -10737, +11063, -11447, -11546, +11900, +11974, +12345, +12345, +12810, +13096, -13325, +13542, +14347, +15346, +15412, -15812, +15872, +15894, +15907, -16049, +16089, -16096, -16125, -16156, -16208, -16303, -16310, -16329, +16436 references
Taq I TCGA +134, +267, +669, +712, +1229, -1413, -2987, +3868, +3899, -3944, +4328, +5125, -5269, +5370, +6049, -7335, -7461, -8005, +9070, -9751, +10084, -10180, +10252, +10893, +11924, -13404, +13635, +14050, +14168, -14956, +15549, +15861, +16178, +16217, +16224, +16238, +16512 references
Xba I TCTAGA -1193, -7440, -8286 references
Xho I CTCGAG -15068 references


Note:

These anaysis have employed numerous endonucleases, including enzymes with four base pair recognition sites, and have been carried out by either digestion of purified mtDNA with separation of the fragments on acrylamide gels (references); or by PCR amplification of the mtDNA in well-defined or overlapping fragments, endonuclease digestion, and separation the restriction fragments on high resolution agarose gels (references) . Each nucleotide listed is the first nucleotide of the restriction endonuclease recognition sequence. A plus (+) indicates a site gain, and a minus (-) indicates a site loss relative to the reference "Cambridge" sequence, Genbank #J01415. Codes for recognition sites are: N=A/G/C/T; P=A/G; ; S=C/G; W=G/T; V=A/C. Restriction sites separated by a slash indicate that the mutation could have occured in one or the other of the listed sites. Restriction analysis and/or sequence analysis of mtDNA extracted from ancient tissues and amplified through PCR is not included in this table but it has been used in several studies (references).