MITOMAP: Mitochondrial DNA Polypeptide Assignments

Last Edited: Mar 09, 2004

Locus	Predicted Mol. Wt.	SDS-PAGE Mobility Tris-glycine Number	SDS-PAGE Mobility Tris-glycine Mol. Wt.	SDS-PAGE Mobility Urea Number	SDS-PAGE Mobility Urea Mol. Wt
MT-ND5	66600	1	43.5	1	51
MT-CO1	57000	2	39.5	23	45
MT-ND4	51400	3	36.5	45	39, 36
MT-CYB	42700	6	27.5	7-10	29
MT-ND2	38900	4	31.5	11	25
MT-ND1	35600	5	29.5	12	24
MT-CO3	30000	8	22.5	15, 16	18
MT-CO2	25500	7	23.6	13, 14	20
MT-ATP6	24800	9	21.6	17, 18	16
MT-ND6	18600	10	16.7	--	--
MT-ND3	13200	12	13.5	23, 24	6
MT-ND4L	10700	11	14.8	26	3.5
MT-ATP8	7900	13+13a	9.8	25	4.5

Note:

Labeling of mitochondrial proteins and gel systems from (references). Mitochondrial proteins are numbered from slowest (#1) to fastest(#13a) mobility. Apparent molecular weights are from (references). Assignments of MTND6(ND6) and MTND3(ND3) from (references) remaining assignments from (references).

Labeling of mitochondrial proteins, gel systems, protein band numbering, and apparent molecular weights from (references). Gene assignments are MTCO1(COI), MTCO2(COII), and MTCO3(COIII) (references) ; MTCO2(COII) and MTATP8(ATPase 8) (references); MTND1(ND1), MTATP6(ATPase6), and MTND3(ND3) (references); MTND4(ND4), MTCYB(Cytb), MTND2(ND2), and MTND4L(ND4L) (references); and MTND6(ND6) (references).