Mitochondrial SNP Primer Selection Tool help

Note: this tool is currently down. It is in the process of being re-written due to our recent move. Please check back next month.

Citing this application:
Poole, Jason; Brandon, Marty 2010. Mitochondrial SNP Primer Selection Tool,


  • Primers are generated using the revised Cambridge Reference Sequence.
  • Select your desired primer length range, melting temperatures, salt and annealing concentrations etc.
  • The position list should be comma separated integers with values ranging from 1 to 16569 and represent the SNP site of interest

Primer Length: Min: Max:
Melting Temperature: Min: Max:
Salt Concentration: [mM]:
Annealing Oligo Concentration: [uM]:
Comma-separated list of mtDNA positions:
(e.g. 3243, 8993, 14459, 14487)

Topic revision: r1 - 12 Feb 2016, MarieLott

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