Primer Selection Help

• Returns primer sequences falling on either side of a SNP site of interest.
• Primers are generated using the revised Cambridge Reference Sequence.
• Select the minimum and maximum length and melting temperatures of primers you are interested in generating.
• You can also select the salt concentration and annealing concentrations or simply leave the default values.
• The position list should be comma separated integers with values ranging from 1 to 16569 and indicative of the positions of interest.